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KMID : 1234420100380010024
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 1 p.24 ~ p.31
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Identification and Antioxidant Activity using Electron Spin Resonance Spectrometry of Antioxidant Producing Marine Actinomycetes Streptomyces sp. ACT-18
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Kim Man-Chul
Kim Ju-Sang
Harikrishnan Ramasamy
Han Yong-Jae
Heo Moon-Soo
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Abstract
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For the research of the natural marine antioxidant, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-18 was gram positive, aerobic, non-motile spores. Substrate mycelia are dark green and yellow gray aerial mycelia. The cell size of the strain was 0.5~1.0 μm. 16S rDNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces sp. Results of cellular fatty acid analysis showed that major cellular fatty acids were C15:0 anteiso (39.33%), C16:1 cis 9 (11.96%), C16:0 (13.08%) and C17:0 anteiso (10.99%). The antioxidant activity of methanol extract from Streptomyce sp. ACT-18 was evaluated by measuring 1,1-diphenyl- 2-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME (Streptomyces Broth Methanol Extract) A-18 was 46% at 0.1 mg/mL. Hydroxyl radical scavenging activity of SBME A-18 was 63% at 0.1 mg/mL. Alkyl radical scavenging activity of SBME A-18 was 39% at 0.1 mg/mL.
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KEYWORD
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Identification, antioxidant activity, marine, Streptomyces, ESR
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